Amber Valley is accessible by rail, with several stations serving the area including Alfreton, Ambergate, Belper, Duffield, Langley Mill and Whatstandwell. Mainline routes run from Edinburgh to London and Liverpool to Norwhich crossing the borough.
Additionally, the Derwent Valley Line operates services between Nottingham and Matlock, offering a scenic journey through the countryside. Passing through the picturesque Derwent Valley Mills World Heritage Site, the line is served by East Midlands Railway (EMR) and operates regular services. The line links to the Ecclesbourne Valley Railway heritage line.